Research Technician (SRI) - Biological Sciences - Regular Full-time 2025-14375

We are seeking an exceptional Research Technician to join our leading-edge neuroscience research team at Sunnybrook Research Institute in Toronto, Canada, in the laboratory of Dr. Isabelle Aubert. This is an outstanding opportunity for a highly skilled and motivated individual to use advanced laboratory techniques to drive innovative discoveries in the preclinical development of brain therapies for neurodegenerative disorders.

At Sunnybrook Research Institute in Toronto, we offer a competitive compensation package, opportunities for professional growth, and a dynamic neuroscience research environment. The salary depends on qualification, starting at $48,750 per year ($25/hour). If you are passionate about working in a lab environment and eager to contribute to groundbreaking neuroscience research, we encourage you to apply. We welcome candidates from all backgrounds and are committed to building a diverse and inclusive team.

To apply, please submit your cover letter, including a statement describing your relevant experience and research interests, your resume, and reference letters from at least two professionals or their contact information. Including reference letters in your application is preferable. Send your application to Dr. Isabelle Aubert (isabelle.aubert@sunnybrook.ca) and Melissa Rahmati (mrahmati@sri.utoronto.ca).

Summary of Duties:

1. Tissue Collection

a.   Perform mouse perfusions, organ harvesting, and brain dissection.

2. Histology and Imaging

a. Prepare, section and stain mouse brain, spinal cord and other tissue for imaging.

b. Conduct immunohistochemistry and RNAscope experiments.

c.  Acquire and analyze images using advanced microscopy (e.g., Leica Stellaris 5 and related software).

3. Molecular Biology

a.   Extract DNA and RNA from tissue specimens.

b.  Perform and analyze qPCR, Western blots, immunoprecipitations, ELISAs.

4. Tissue Culture

a.   Primary culture of oligodendrocytes and neural stem cells.

b.  Prepare the necessary growth medium and supplies.

5. General

a. Maintain detailed records and documentation of experiments, procedures and results.

b.  Comply with laboratory safety protocols (BSL2) and ethical standards.    

c. Expertise in using Microsoft Office, image editing software, and statistical tools, particularly GraphPad Prism.

d.  Knowledge of the brain anatomy, neuroscience, and disorders of aging

Qualifications:

• Master of Science in Neuroscience, Biology, Biomedical Engineering or a related field.
• Proficiency in histology, including brain tissue processing, cryostat or microtome sectioning, staining and slide preparation.
• Expertise in immunohistochemistry and RNAscope techniques, particularly in rodent models.
• Hands-on experience with tissue culture methods.
• Strong problem-solving and analytical skills.